| 326 | The Willink School | 1,233 pupils | State · Day · 6th | Reading, South East | — |
| 327 | Heathside School | 1,230 pupils | Academy · Day · 6th | Weybridge, South East | — |
| 328 | The Cooper School | 1,228 pupils | Academy · Day · 6th | Bicester, South East | — |
| 329 | St Anne's Catholic School | 1,225 pupils | Academy · Day · 6th | Southampton, South East | — |
| 330 | St Birinus School | 1,212 pupils | Academy · Day · 6th | Didcot, South East | — |
| 331 | The Thomas Aveling School | 1,212 pupils | Academy · Day · 6th | Rochester, South East | — |
| 332 | Simon Langton Grammar School for Boys | 1,211 pupils | Grammar · Day · 6th | Canterbury, South East | — |
| 333 | The Radcliffe School | 1,210 pupils | State · Day · 6th | Milton Keynes, South East | — |
| 334 | Ash Manor School | 1,209 pupils | State · Day | Ash, South East | — |
| 335 | Woking High School | 1,208 pupils | Academy · Day | Woking, South East | — |
| 336 | The Bishop Wand Church of England School | 1,200 pupils | Academy · Day · 6th | Sunbury-on-Thames, South East | — |
| 337 | Ratton School | 1,197 pupils | Academy · Day | Eastbourne, South East | — |
| 338 | Cowes Enterprise College, An Ormiston Academy | 1,192 pupils | Academy · Day · 6th | Cowes, South East | — |
| 339 | Guildford County School | 1,190 pupils | Academy · Day · 6th | Guildford, South East | — |
| 340 | Wexham School | 1,188 pupils | State · Day · 6th | Slough, South East | — |
| 341 | Slough and Eton Church of England Business and Enterprise College | 1,178 pupils | Academy · Day · 6th | Slough, South East | — |
| 342 | Wilmington Grammar School for Boys | 1,178 pupils | Grammar · Day · 6th | Dartford, South East | — |
| 343 | St Gregory's Catholic School | 1,177 pupils | Academy · Day · 6th | Tunbridge Wells, South East | — |
| 344 | St Wilfrid's Catholic Comprehensive School, Crawley | 1,174 pupils | State · Day · 6th | Crawley, South East | — |
| 345 | Perins School | 1,166 pupils | Academy · Day | Alresford, South East | — |
| 346 | Priory School | 1,163 pupils | State · Day | Lewes, South East | — |
| 347 | St Simon Stock Catholic School | 1,151 pupils | Academy · Day · 6th | Maidstone, South East | — |
| 348 | St Edmund's Catholic School | 1,150 pupils | State · Day | Portsmouth, South East | — |
| 349 | Claverham Community College | 1,148 pupils | State · Day | Battle, South East | — |
| 350 | Wheatley Park School | 1,146 pupils | Academy · Day · 6th | Oxford, South East | — |