| 1576 | The Hackthorn Church of England Primary School | 56 pupils | State · Day | Lincoln, East Midlands | — |
| 1577 | Faldingworth Community Primary School | 55 pupils | State · Day | Market Rasen, East Midlands | — |
| 1578 | Weston St Mary Church of England Primary School | 54 pupils | Academy · Day | Spalding, East Midlands | — |
| 1579 | Wigley Primary School | 53 pupils | State · Day | Chesterfield, East Midlands | — |
| 1580 | Long Clawson Church of England Primary School | 52 pupils | State · Day | Melton Mowbray, East Midlands | — |
| 1581 | Normanby Primary School | 52 pupils | State · Day | Market Rasen, East Midlands | — |
| 1582 | St Hardulph's Church of England Primary School | 50 pupils | State · Day | Derby, East Midlands | — |
| 1583 | Fitzherbert CofE (Aided) Primary School | 49 pupils | State · Day | Ashbourne, East Midlands | — |
| 1584 | Monyash CofE Primary School | 49 pupils | State · Day | Bakewell, East Midlands | — |
| 1585 | North Scarle Primary School | 49 pupils | State · Day | Lincoln, East Midlands | — |
| 1586 | Boddington Church of England Primary Academy | 48 pupils | Academy · Day | Daventry, East Midlands | — |
| 1587 | Hague Bar Primary School | 47 pupils | Academy · Day | High Peak, East Midlands | — |
| 1588 | Hollycroft Primary School | 47 pupils | Free School · Day | HINCKLEY, East Midlands | — |
| 1589 | Hose Church of England Primary School | 47 pupils | State · Day | Melton Mowbray, East Midlands | — |
| 1590 | Litton CofE Primary School | 46 pupils | State · Day | Buxton, East Midlands | — |
| 1591 | Cosgrove Village Primary School | 45 pupils | State · Day | Cosgrove, East Midlands | — |
| 1592 | Matlock Bath Holy Trinity CofE Controlled Primary School | 44 pupils | State · Day | Matlock, East Midlands | — |
| 1593 | Willoughton Primary School | 44 pupils | State · Day | Gainsborough, East Midlands | — |
| 1594 | Somerby Primary School | 43 pupils | Academy · Day | Melton Mowbray, East Midlands | — |
| 1595 | Tiffield Church of England Voluntary Aided Primary School | 43 pupils | State · Day | Towcester, East Midlands | — |
| 1596 | The Holbeach St Mark's Church of England Primary School | 42 pupils | State · Day | Spalding, East Midlands | — |
| 1597 | Croxton Kerrial Church of England Primary School | 41 pupils | Academy · Day | Grantham, East Midlands | — |
| 1598 | Kirk Ireton C of E Primary School | 40 pupils | State · Day | Ashbourne, East Midlands | — |
| 1599 | St Margaret's Catholic Voluntary Academy | 40 pupils | Academy · Day | Glossop, East Midlands | — |
| 1600 | Crich Carr CofE Primary School | 38 pupils | State · Day | Matlock, East Midlands | — |