| 1001 | Prae Wood Primary School | 448 pupils | State · Day | St Albans, East of England | — |
| 1002 | Highwoods Community Primary School | 447 pupils | Academy · Day | Colchester, East of England | — |
| 1003 | Tudor Primary School | 447 pupils | State · Day | Hemel Hempstead, East of England | — |
| 1004 | Isle of Ely Primary School | 446 pupils | Academy · Day | Ely, East of England | — |
| 1005 | Kingswood Primary School and Nursery | 446 pupils | State · Day | Basildon, East of England | — |
| 1006 | Lordship Farm Primary School | 446 pupils | State · Day | Letchworth Garden City, East of England | — |
| 1007 | Tithe Farm Primary School | 446 pupils | State · Day | Dunstable, East of England | — |
| 1008 | Somers Heath Primary School | 445 pupils | Academy · Day | South Ockendon, East of England | — |
| 1009 | Charles Darwin Primary School | 443 pupils | Free School · Day | Norwich, East of England | — |
| 1010 | Peckover Primary School | 443 pupils | Academy · Day | Wisbech, East of England | — |
| 1011 | Russell Lower School | 443 pupils | State · Day | Ampthill, East of England | — |
| 1012 | Summercroft Primary School | 443 pupils | Academy · Day | Bishop's Stortford, East of England | — |
| 1013 | St Edmund's Catholic Primary School | 442 pupils | State · Day | Bury St Edmunds, East of England | — |
| 1014 | Leagrave Primary School | 441 pupils | State · Day | Luton, East of England | — |
| 1015 | Wormley CofE Primary School | 441 pupils | State · Day | Broxbourne, East of England | — |
| 1016 | Bonnygate Primary School | 440 pupils | Academy · Day | South Ockendon, East of England | — |
| 1017 | Wentworth Primary School | 440 pupils | State · Day | Maldon, East of England | — |
| 1018 | Howe Dell Primary School | 439 pupils | State · Day | Hatfield, East of England | — |
| 1019 | Kings Langley Primary School | 439 pupils | State · Day | Kings Langley, East of England | — |
| 1020 | The Bishops' Church of England and Roman Catholic Primary School | 439 pupils | State · Day | Chelmsford, East of England | — |
| 1021 | The Gateway Primary Free School | 439 pupils | Free School · Day | Grays, East of England | — |
| 1022 | Cuffley School | 438 pupils | State · Day | Potters Bar, East of England | — |
| 1023 | Leverton Primary School | 438 pupils | State · Day | Waltham Abbey, East of England | — |
| 1024 | Old Fletton Primary School | 438 pupils | State · Day | Peterborough, East of England | — |
| 1025 | Hazeldene School | 437 pupils | State · Day | Bedford, East of England | — |